diff --git a/changelog.d/10063.removal b/changelog.d/10063.removal
new file mode 100644
index 0000000000000000000000000000000000000000..0f8889b6b4d72fd1de46f6bd1a6399741d46e5cf
--- /dev/null
+++ b/changelog.d/10063.removal
@@ -0,0 +1 @@
+Remove the experimental `spaces_enabled` flag. The spaces features are always available now.
diff --git a/docs/sample_config.yaml b/docs/sample_config.yaml
index 6576b153d0eb2c587243a3b98986a62628b06a08..7b97f73a296bd79b1af43af252ec7084c0b642d7 100644
--- a/docs/sample_config.yaml
+++ b/docs/sample_config.yaml
@@ -2916,18 +2916,3 @@ redis:
   # Optional password if configured on the Redis instance
   #
   #password: <secret_password>
-
-
-# Enable experimental features in Synapse.
-#
-# Experimental features might break or be removed without a deprecation
-# period.
-#
-experimental_features:
-  # Support for Spaces (MSC1772), it enables the following:
-  #
-  # * The Spaces Summary API (MSC2946).
-  # * Restricting room membership based on space membership (MSC3083).
-  #
-  # Uncomment to disable support for Spaces.
-  #spaces_enabled: false
diff --git a/synapse/api/room_versions.py b/synapse/api/room_versions.py
index c9f9596ada9225e1d2c4381e36ab52c2fc32f3de..373a4669d019e429dd1f2f0ef344863d7516f904 100644
--- a/synapse/api/room_versions.py
+++ b/synapse/api/room_versions.py
@@ -181,6 +181,6 @@ KNOWN_ROOM_VERSIONS = {
         RoomVersions.V5,
         RoomVersions.V6,
         RoomVersions.MSC2176,
+        RoomVersions.MSC3083,
     )
-    # Note that we do not include MSC3083 here unless it is enabled in the config.
 }  # type: Dict[str, RoomVersion]
diff --git a/synapse/config/experimental.py b/synapse/config/experimental.py
index cc67377f0fe780e5bbdefd1d497415aebcc079d4..6ebce4b2f75d4c5e1716192e0b9cff3128d85ce9 100644
--- a/synapse/config/experimental.py
+++ b/synapse/config/experimental.py
@@ -12,7 +12,6 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 
-from synapse.api.room_versions import KNOWN_ROOM_VERSIONS, RoomVersions
 from synapse.config._base import Config
 from synapse.types import JsonDict
 
@@ -28,27 +27,5 @@ class ExperimentalConfig(Config):
         # MSC2858 (multiple SSO identity providers)
         self.msc2858_enabled = experimental.get("msc2858_enabled", False)  # type: bool
 
-        # Spaces (MSC1772, MSC2946, MSC3083, etc)
-        self.spaces_enabled = experimental.get("spaces_enabled", True)  # type: bool
-        if self.spaces_enabled:
-            KNOWN_ROOM_VERSIONS[RoomVersions.MSC3083.identifier] = RoomVersions.MSC3083
-
         # MSC3026 (busy presence state)
         self.msc3026_enabled = experimental.get("msc3026_enabled", False)  # type: bool
-
-    def generate_config_section(self, **kwargs):
-        return """\
-        # Enable experimental features in Synapse.
-        #
-        # Experimental features might break or be removed without a deprecation
-        # period.
-        #
-        experimental_features:
-          # Support for Spaces (MSC1772), it enables the following:
-          #
-          # * The Spaces Summary API (MSC2946).
-          # * Restricting room membership based on space membership (MSC3083).
-          #
-          # Uncomment to disable support for Spaces.
-          #spaces_enabled: false
-        """
diff --git a/synapse/federation/transport/server.py b/synapse/federation/transport/server.py
index 9d50b05d0152a0882088919341c0669763d74ceb..00ff02c7cbb5b3ad6c4e50a6793a664b051e249d 100644
--- a/synapse/federation/transport/server.py
+++ b/synapse/federation/transport/server.py
@@ -1562,13 +1562,12 @@ def register_servlets(
                 server_name=hs.hostname,
             ).register(resource)
 
-        if hs.config.experimental.spaces_enabled:
-            FederationSpaceSummaryServlet(
-                handler=hs.get_space_summary_handler(),
-                authenticator=authenticator,
-                ratelimiter=ratelimiter,
-                server_name=hs.hostname,
-            ).register(resource)
+        FederationSpaceSummaryServlet(
+            handler=hs.get_space_summary_handler(),
+            authenticator=authenticator,
+            ratelimiter=ratelimiter,
+            server_name=hs.hostname,
+        ).register(resource)
 
     if "openid" in servlet_groups:
         for servletclass in OPENID_SERVLET_CLASSES:
diff --git a/synapse/rest/client/v1/room.py b/synapse/rest/client/v1/room.py
index 51813cccbe72eb79b6edc52ae4c3902ee602f8fa..d6d55893af8a1f91b72aa650b514740bf5686835 100644
--- a/synapse/rest/client/v1/room.py
+++ b/synapse/rest/client/v1/room.py
@@ -1060,9 +1060,7 @@ def register_servlets(hs: "HomeServer", http_server, is_worker=False):
     RoomRedactEventRestServlet(hs).register(http_server)
     RoomTypingRestServlet(hs).register(http_server)
     RoomEventContextServlet(hs).register(http_server)
-
-    if hs.config.experimental.spaces_enabled:
-        RoomSpaceSummaryRestServlet(hs).register(http_server)
+    RoomSpaceSummaryRestServlet(hs).register(http_server)
 
     # Some servlets only get registered for the main process.
     if not is_worker: